85 Construction of recombinant 85 C antigen of mycobacterium
leprae in E. coli
Title:
85 Construction of recombinant 85 C antigen of mycobacterium
leprae in E.coli.
Author:
Yin Y, Wu Q, et al.
Address:
National Center for STD and Leprosy Control, Institute of
Dermatology, Chinese Academy of Medical Sciences, 12 Jiangwangmiao
Road, Nanjing 210042, P.R.China
Source:
Chinese Journal of Dermatology, 1996 (29) 5: 343-345
Abstract:
Objective: screen an antigen with high
specificity and immunity for the serodiagnosis and explore
the etiology of leprosy. Methods: 85C antigen
of mycobacterium leprae was amplified by PCR technique,
and insert amplified fragment into expression vector. Results:
The recombinany 85C protein was recognized by anti
-a-TB antisera. Conclusions: The result
of this study will be useful for the further study of a-antigen
Key words: mycobacterium leprae; 85C antigen
Language: Chinese
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