Study on method of expression for recombinant a1 and a2 antigen of M. leprae

Title:
Study on method of expression for recombinant a1 and a2 antigen of M. leprae

Author:
Yin Y, Wu Q, Hou W

Address:
National Center for STD and Leprosy Control, Institute of Dermatology, Chinese Academy of Medical Sciences, 12 Jiangwangmiao Road, Nanjing 210042, P. R. China

Source:
China leprosy journal,1998（14）2:79-81

Abstract:
OBJECTIVES: Study on method of expression for recombinant a1 and a2 antigen of M. leprae. METHODS: The recombinant a antigen was efficiently expressed on E. Coli by using pMALc-RI as expression vector, and purified by amylose resin affinity chromatography. RESULTS: More than 6 mg and 10mg recombinant a1 and a2 proteins were obtained from 200ml liquid culture respectively. CONCLUSION: The pMALc-RI was a efficiently expression vector for recombinant a1 and a2 antigen of M. leprae.

Key words: M. leprae antigen

Language: Chinese