Praparation of a2 Antigen of ML in E. Coli and the application for sero-diagnosis of leprosy

Title:
Praparation of a2 Antigen of ML in E. Coli and the application for sero-diagnosis of leprosy

Author:
Yin Y, Suzuki Y, Makino M, Wu Q

Address:
National Center for STD and Leprosy Control, Institute of Dermatology, Chinese Academy of Medical Sciences, 12 Jiangwangmiao Road, Nanjing 210042, P. R. China

Source:
Chinese medical sciences journal,1999（14）2:106-107

Abstract:
OBJECTIVES: The recombinant a2 antigen of M. leprae was prepared using the molecular biologic tools and the recombinant DNA expression technology. METHODS: Screening of the M. leprae expression library was performed by the plaque hybridization technique Nucleotide sequences were determined by dideoxy termination method. RESULTS: The gene coding for a2 antigen of M. leprae was cloned characterized, and the complete nucleotide sequence data has been assigned in the GSDB, DDBJ, EMBJ, EMBL AND NCBI nucleotide sequence databank. The over expression system of a2 antigen gene in E. coli was constructed, and the recombinant a2 antigen has been purified by amylase column chromatography at the purity of more than 95%. CONCLUSION: The recombinant a2 antigene of M. leprae could be used as one of the specific antigens for the sero-diagnosis of leprosy.

Key words: Mycbacterium leprae; gene cloning; recombinant antigen

Language: English