Gene cloning and expression of mycobacterium leprae a2
antigen
Title:
Gene cloning and expression of mycobacterium leprae a2antigen
Author:
Yin Y, Suzuki Y, Makino M, Wu Q
Address:
National Center for STD and Leprosy Control, Institute
of Dermatology, Chinese Academy of Medical Sciences, 12
Jiangwangmiao Road, Nanjing 210042, P. R. China
Source:
Acta academiae medicinae sinicaeŁ¬1999 (21) 1: 62-67
Abstract:
OBJECTIVES: The recombinant a2 antigen
of M. leprae was prepared using the molecular biologic
tools and the recombinant DNA expression technology. METHODS:
Screening of the M. eprae expression library
was performed by the plaque hybridization technique Nucleotide
sequences were determined by dideoxy termination method.
RESULTS: The gene coding for a2 antigen
of M. leprae was cloned characterized, and the complete
nucleotide sequence data has been assigned in the GSDB,
DDBJ, EMBJ, EMBL AND NCBI nucleotide sequence databank.
The over expression system of a2 antigen gene in E. coli
was constructed, and the recombinant a2 antigen has been
purified by amylose column chromatography at the purity
of more than 95%. CONCLUSION: The recombinant
a2 antigene of M. leprae could be used as one of the specific
antigens for the sero-diagnosis of leprosy.
Key words: Mycbacterium leprae; gene
cloning; recombinant antigen
Language: Chinese
