Study on Detection of IgM Antibody Against Mycobacterium Leprae in Mice Sera with ...

Title:
Study on Detection of IgM Antibody Against Mycobacterium Leprae in Mice Sera with ND-O-BSA-ELISA

Author:
Wang H, Zhang W, Wu Q, et al

Address:
National Center for STD and Leprosy Control, Institute of Dermatology, Chinese Academy of Medical Sciences, 12 Jiangwangmiao Road, Nanjing 210042, P. R. China

Source:
Chinese Journal of Dermatology 1999, 32 (3):168-170

Abstract:
OBJECTIVES: To reveal the variation of specific IgM type antibodies in the mice infected by M. leprae. METHODS: The serum level of IgM antibody against M. leprae from 50 normal mice and 127 mice infected with M. leprae were detected with ND-O-BSA-ELISA. RESULTS: The serum level of IgM antibody against M. leprae in infected mice was significantly higher than that in normal mice(P<0.01). There were significant differences between untreated group of mice and treated group of mice (P<0.01) between different drug-treated groups (P<0.01),and between different dosage groups in the same drug-treated mice (P<0.01 or P<0.05). CONCLUSION: The serum level of IgM antibody against M. leprae in infected mice is consistent with bacterial load and related to the severity of infection and the therapeutical effects.

Key words: ND-O-BSA-ELISA Mycobacterium Leprae Serology

Language: Chinese