Gene application test of Mycobacterium Leprae in paraffin-embedded tissue

Title:
Gene application test of Mycobacterium Leprae in paraffin-embedded tissue

Author:
Wu Q, Yin Y, Zhang L, Yu Y, Hou W, Ye G

Address:
National Center for STD and Leprosy Control, Institute of Dermatology, Chinese Academy of Medical Sciences, 12 Jiangwangmiao Road, Nanjing 210042, P. R. China

Source:
Chin J Dermatol. 2000, 33(S-1): 63-64

Abstract:
Objective: To establish polymerase chain reaction (PCR) using DNA of M. leprae from fixed and paraffin-embedded tissue(FPET). Methods: The DNA of M. leprae to be released from FPET and purified by using Texpat Kit and 100% alcohol, then the PCR was conducted. Results: 32 samples, BI of which were all more than 1+, 28/32 positive reactions were yielded, 1 sample, its BI was equal to zero, negative result was yielded. The sensitivity of PCR reached 0.04pg of DNA level. Conclusion: This method is very useful to amplify the DNA of M. leprae from fixed and paraffin embedded tissue.

Key words: Leprosy PCR

Language: Chinese