Three year follows-up results of detecting IgM antibody
against ND-O-BSA in ...
Title:
Three year follows-up results of detecting IgM antibody
against ND-O-BSA in sera from leprosy patients cured after
DDS monotherapy
Author:
Wu Q, Yin Y, Zhang L, et al.
Address:
National Center for STD and Leprosy Control, Institute
of Dermatology, Chinese Academy of Medical Sciences, 12
Jiangwangmiao Road, Nanjing 210042, P. R. China
Source:
Acta Academiae Medicinae sinicae, 2000 22(4):375-387
Abstract:
Objective: To understand the possibility
for detecting and predicting the relapses in leprosy using
a serological tool. Methods: Following-up
the leprosy patients cured after DDS monotherapy using
a ND-O-BSA-based ELISA£¨ND-ELISA). Detecting the trends
of results of relapses in leprosy. The analyses were statistically
conducted with corresponding statistical methods such
as x¡À3s, RR(risk ratio), AR(attributable risk) and so
on. Results: A total of 666 sera that
were collected from leprosy patients cured after DDS monotherapy.
Of whom 430 were diagnosed as MB cases before DDS monotherapy
(i.e. post DDS MB, P-MB), and others were diagnosed as
PB cases (i.e. post DDS PB, P-PB). All of them were periodically
determined the levels of IgM antibody in their sera using
ND-ELISA. The results indicated that, 1. In P-MB, of whom
95 were positive, and 12 of them were diagnosed as relapses
in leprosy, additional 335 cases were negative, and 1
of them was diagnosed as relapse in leprosy; In P-PB,
of whom 44 were positive and 192 were negative one case
of relapse in leprosy in both to be found, respectively.
In 666 cases of P-MB and P-PB, a total of 5 cases relapse
in leprosy to be found. The risk of relapse was higher
6.7 times in P-MB than that in P-PB. 2. In IgM antibody
positive group, the accumulative rate of relapse was 13.68%,
In IgM antibody negative group, it was 0.35%. Their RR
and AR were 36.7 and 13.33%, respectively. 3. In relapsed
cases, in the majority of them, their levels of IgM antibody
were increased gradually, or positive consistently from
curing to relapse. Meanwhile, the levels of IgM antibody
in relapsed leprosy were gradually decreased with effective
treatment. 4. The time of relapse in leprosy was 1-2 years(>2
years in the majority of them ) after levels of IgM antibody
determined became positivity. As for the incubation time
of relapse was more than 10 years in the majority of relapsed
leprosy after their relapse from treatment. No clustered
period of years to be found. 5. In all of relapsed leprosy,
only one case that shown the change of type from TT to
BT after relapsing in leprosy. Conclusion:
Above results suggested that using ND-ELISA for determining
and following-up the levels of IgM antibody in individuals
cured after discontinuation of treatment was valuable
to case-finding of relapsed leprosy and predicting the
risk of relapse in leprosy.
Key words: leprosy; relapse; ELISA
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